Phosphoproteomic analysis of the response to DNA damage in Trypanosoma brucei.

ABSTRACT: Damage to the genetic material of the cell poses a universal threat to all forms of life. The DNA damage response is a coordinated cellular response to a DNA break, key to which is the phosphorylation signaling cascade. Identifying which proteins are phosphorylated is therefore crucial to understanding the mechanisms that underlie it. We have used stable isotopic labeling of amino acids in cell culture-based quantitative phosphoproteomics to profile changes in phosphorylation site abundance following double stranded DNA breaks, at two distinct loci in the genome of the single cell eukaryote Trypanosoma brucei. Here, we report on the T. brucei phosphoproteome following a single double-strand break at either a chromosome internal or subtelomeric locus, specifically the bloodstream form expre

SUBMITTER: McLaughlin E 

PROVIDER: S-EPMC11408851 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature