Genomic insights into epidemic plasmids carrying bla _CTX-M and mcr-1 genes in Escherichia coli from Lebanese broiler production.

ABSTRACT:

Background

In a previous nationwide survey in the Lebanese broiler production, multidrug-resistant CTX-M-producing E. coli were found to carry the mobile colistin resistance gene mcr-1.

Objectives

To investigate the mobile genetic supports responsible for the spread of these resistance genes among E. coli in healthy broilers in Lebanon.

Methods

Thirty-three bla _CTX-M and mcr-1 positive E. coli of various sequence types from 17 broilers farms were subjected to conjugation assays. Long-read sequencing (Oxford Nanopore Technologies) and hybrid assembly were performed to determine complete plasmid sequences and their phylogenetic diversity.

Results

Twenty-nine conjugative IncFII plasmids harboured the exte

SUBMITTER: Mikhayel M 

PROVIDER: S-EPMC11408927 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature