Cannabinoid type 2 receptor deficiency leads to Aβ-induced cognitive impairment through promoting microglial sensitivity to Aβ in the prefrontal cortex in mice.

ABSTRACT:

Aims

This study is to investigate the effects of Cannabinoid type 2 receptor (CB₂R) deficiency on microglia and cognitive function in both Aβ_1-42-injected CB₂R knockout mice and a transgenic mouse model of Alzheimer's disease (AD) in brain.

Methods

After hippocampal injection with Aβ_1-42 oligomers in CB₂R knockout mice with and without CB₂R agonist treatment and in transgenic APP/PS1 mice with CB₂R deletion, the novel object recognition (NOR) and Morris water maze (MWM) tests were performed to assess the animal behavior performance. Immunofluorescence staining was conducted to detect the microglial morphology and activation status. The expression of proinflammation and anti-inflammation cytokines we

SUBMITTER: Zhang T 

PROVIDER: S-EPMC11409075 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature