Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry.

ABSTRACT:

Introduction

Phages are viruses that infect prokaryotes and can shape microbial communities by lysis, thus offering applications in various fields. However, challenges exist in sampling, isolation and accurate prediction of the host specificity of phages as well as in the identification of newly replicated virions in response to environmental challenges.

Methods

A new workflow using biorthogonal non-canonical amino acid tagging (BONCAT) and click chemistry (CC) allowed the combined analysis of phages and their hosts, the identification of newly replicated virions, and the specific tagging of phages with biotin for affinity chromatography.

Results

Replication of phage λ in Escherichia coli was selected as a model for workflow development. Specific labeling of ph

SUBMITTER: Hellwig P 

PROVIDER: S-EPMC11409252 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature