Metabolic features of neutrophilic differentiation of HL-60 cells in hyperglycemic environments.

ABSTRACT:

Introduction

Chronic hyperglycemia affects neutrophil functions, leading to reduced pathogen killing and increased morbidity. This impairment has been directly linked to increased glycemia, however, how this specifically affects neutrophils metabolism and their differentiation in the bone marrow is unclear and difficult to study.

Research design and methods

We used high-resolution respirometry to investigate the metabolism of resting and activated donor neutrophils, and flow cytometry to measure surface CD15 and CD11b expression. We then used HL-60 cells differentiated towards neutrophil-like cells in standard media and investigated the effect of doubling glucose concentration on differentiation metabolism. We measured the oxygen consumption rate (OCR), and the enzymatic act

SUBMITTER: Cazares-Preciado JA 

PROVIDER: S-EPMC11409339 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature