Regression of renal cell carcinoma by T cell receptor-engineered T cells targeting a human endogenous retrovirus.

ABSTRACT:

Background

We discovered a novel human endogenous retrovirus (CT-RCC HERV-E) that was selectively expressed in most clear cell renal cell carcinomas (ccRCC) and served as a source of antigens for T cell-mediated killing. Here, we described the cloning of a novel T cell receptor (TCR) targeting a CT-RCC HERV-E-derived antigen specific to ccRCC and characterized antitumor activity of HERV-E TCR-transduced T cells (HERV-E T cells).

Methods

We isolated a CD8⁺ T cell clone from a patient with immune-mediated regression of ccRCC post-allogeneic stem cell transplant that recognized the CT-RCC-1 HERV-E-derived peptide in an HLA-A11-restricted manner. We used 5'Rapid Amplification of cDNA Ends (RACE) to clone the full length HERV-E TCR and generated retrovirus encoding thi

SUBMITTER: Barisic S 

PROVIDER: S-EPMC11409391 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature