Sequence-based engineering of pH-sensitive antibodies for tumor targeting or endosomal recycling applications.

ABSTRACT: The engineering of pH-sensitive therapeutic antibodies, particularly for improving effectiveness and specificity in acidic solid-tumor microenvironments, has recently gained traction. While there is a justified need for pH-dependent immunotherapies, current engineering techniques are tedious and laborious, requiring repeated rounds of experiments under different pH conditions. Inexpensive computational techniques to predict the effectiveness of His pH-switches require antibody-antigen complex structures, but these are lacking in most cases. To circumvent these requirements, we introduce a sequence-based in silico method for predicting His mutations in the variable region of antibodies, which could lead to pH-biased antigen binding. This method, called Sequence-based Identification o

SUBMITTER: Wei W 

PROVIDER: S-EPMC11409498 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature