A growth-based screening strategy for engineering the catalytic activity of an oxygen-sensitive formate dehydrogenase.

ABSTRACT: Enzyme engineering is a powerful tool for improving or altering the properties of biocatalysts for industrial, research, and therapeutic applications. Fast and accurate screening of variant libraries is often the bottleneck of enzyme engineering and may be overcome by growth-based screening strategies with simple processes to enable high throughput. The currently available growth-based screening strategies have been widely employed for enzymes but not yet for catalytically potent and oxygen-sensitive metalloenzymes. Here, we present a screening system that couples the activity of an oxygen-sensitive formate dehydrogenase to the growth of Escherichia coli. This system relies on the complementation of the E. coli formate hydrogenlyase (FHL) complex by Mo-dependent formate dehyd

SUBMITTER: Li F 

PROVIDER: S-EPMC11409667 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature