A promoter-RBS library for fine-tuning gene expression in Methanosarcina acetivorans.

ABSTRACT: Methanogens are the main biological producers of methane on Earth. Methanosarcina acetivorans is one of the best characterized methanogens that has powerful genetic tools for genome editing. To study the physiology of this methanogen in further detail as well as to effectively balance the flux of their engineered metabolic pathways in expansive project undertakings, there is the need for controlled gene expression, which then requires the availability of well-characterized promoters and ribosome-binding sites (RBS). In this study, we constructed a library of 33 promoter-RBS combinations that includes 13 wild-type and 14 hybrid combinations, as well as six combination variants in which the 5'-untranslated region (5'UTR) was rationally engineered. The expression strength for each comb

SUBMITTER: Zhu P 

PROVIDER: S-EPMC11409679 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature