The tal gene of lactococcal bacteriophage TP901-1 is involved in DNA release following host adsorption.

ABSTRACT: Temperate P335 phage TP901-1 represents one of the best-characterized Gram-positive phages regarding its structure and host interactions. Following its reversible adsorption to the polysaccharidic side-chain of the cell wall polysaccharide of its host Lactococcus cremoris 3107, TP901-1 requires a glucosylated cell envelope moiety to trigger its genome delivery into the host cytoplasm. Here, we demonstrate that three distinct single amino acid substitutions in the Tal protein of TP901-1 baseplate are sufficient to overcome the TP901-1 resistance of three L. cremoris 3107 derivatives, whose resistance is due to impaired DNA release of the phage. All of these Tal alterations are located in the N-terminally located gp27-like domain of the protein, conserved in many tailed phages.

SUBMITTER: Ruiz-Cruz S 

PROVIDER: S-EPMC11409707 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature