Fluorescence-plane polarization for the real-time monitoring of transferase migration in living cells.

ABSTRACT: Transferases are enzymes that exhibit multisite migration characteristics. Significantly, enzyme activity undergoes changes during this migration process, which inevitably impacts the physiological function of living organisms and can even lead to related malignant diseases. However, research in this field has been severely hindered by the lack of tools for the simultaneous and differential monitoring of site-specific transferase activity. Herein, we propose a novel strategy that integrates a fluorescence signal response with high sensitivity and an optical rotation signal response with superior spatial resolution. To validate the feasibility of this strategy, transferase γ-glutamyltransferase (GGT) was used as a model system to develop dual-mode chiral probes ACx-GGTB (AC17-GGTB an

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11409853 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature