Genome assembly of Stephania longa provides insight into cepharanthine biosynthesis.

ABSTRACT:

Introduction

Stephania longa, a medicinal plant renowned for producing cepharanthine, has gained significance due to the compound's notable antiviral properties against SARS-CoV-2. However, a comprehensive genetic understanding of S. longa has been lacking. This study aimed to develop a high-quality, chromosome-level genome assembly to uncover the genetic intricacies and evolutionary narrative of this species. By integrating genomic data with metabolomic and transcriptomic analyses, we sought to identify key genes involved in cepharanthine biosynthesis.

Methods

We employed a multi-faceted approach comprising genome assembly, phylogenetic analysis, gene family dynamics investigation, metabolomic profiling, and gene expression analysis across various tissues of S. lo

SUBMITTER: Shang H 

PROVIDER: S-EPMC11410628 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature