HOXA9 promotes proliferation, metastasis and prevents apoptosis in hepatocellular carcinoma.

ABSTRACT:

Purpose

This research aimed to evaluate the expression level of Homeobox A9 (HOXA9) and its role in tumorigenesis of hepatocellular carcinoma (HCC).

Methods

Bioinformatic analysis, qPCR and Western blot analysis of clinical samples were employed to evaluate mRNA and protein levels of HOXA9 in HCC patients and cell lines. In vitro cell proliferation, migration and invasion, cloning formation, xenograft tumor model, wound healing and apoptosis assays, RNA sequencing analysis of RPL38-silenced HCC-LM3 cells and qPCR, Western blot analysis were performed for validation. Analysis of HOXA9-related genes were conducted to identify their relationships between HOXA9.

Results

HOXA9 is dramatically upregulated in HCC. Upregulation of HOXA9 in HCC predicts poor survival of patien

SUBMITTER: Bao G 

PROVIDER: S-EPMC11410850 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature