Non-invasive quantification of stem cell-derived islet graft size and composition.

ABSTRACT:

Aims/hypothesis

Stem cell-derived islets (SC-islets) are being used as cell replacement therapy for insulin-dependent diabetes. Non-invasive long-term monitoring methods for SC-islet grafts, which are needed to detect misguided differentiation in vivo and to optimise their therapeutic effectiveness, are lacking. Positron emission tomography (PET) has been used to monitor transplanted primary islets. We therefore aimed to apply PET as a non-invasive monitoring method for SC-islet grafts.

Methods

We implanted different doses of human SC-islets, SC-islets derived using an older protocol or a state-of-the-art protocol and SC-islets genetically rendered hyper- or hypoactive into mouse calf muscle to yield different kinds of grafts. We followed the grafts with PET using two tracer

SUBMITTER: Lithovius V 

PROVIDER: S-EPMC11410899 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature