Toxoplasma FER1 is a versatile and dynamic mediator of differential microneme trafficking and microneme exocytosis.

ABSTRACT: Toxoplasma gondii is a polarized cell concentrating several secretory organelles at the apical pole. The secretory micronemes come in two sub-populations differentiated by dependence on Rab5A/C in their biogenesis. Calcium-dependent exocytosis of micronemes occurs at the very apical tip and is critical for parasite egress from its host cell, adhesion and invasion of the next cell. Ferlins represent a protein family with roles in exocytosis containing multiple Ca²⁺-sensing C2 domains. We determined that T. gondii's ferlin 1 (FER1) localized dynamically to the parasite's secretory pathway. FER1 function was dissected by dominant negative overexpression strategies. We demonstrated that FER1 traffics microneme organelles along the following trajectories: (1) Along the cortex to the

SUBMITTER: Tagoe DNA 

PROVIDER: S-EPMC11410953 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature