Low-frequency CD8⁺ T cells induced by SIGN-R1⁺ macrophage-targeted vaccine confer SARS-CoV-2 clearance in mice.

ABSTRACT: Vaccine-induced T cells and neutralizing antibodies are essential for protection against SARS-CoV-2. Previously, we demonstrated that an antigen delivery system, pullulan nanogel (PNG), delivers vaccine antigen to lymph node medullary macrophages and thereby enhances the induction of specific CD8⁺ T cells. In this study, we revealed that medullary macrophage-selective delivery by PNG depends on its binding to a C-type lectin SIGN-R1. In a K18-hACE2 mouse model of SARS-CoV-2 infection, vaccination with a PNG-encapsulated receptor-binding domain of spike protein decreased the viral load and prolonged the survival in the CD8⁺ T cell- and B cell-dependent manners. T cell receptor repertoire analysis revealed that although the vaccine induced T cells at various frequencies

SUBMITTER: Muraoka D 

PROVIDER: S-EPMC11411095 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature