In vitro differentiation of mouse pluripotent stem cells into corticosteroid-producing adrenocortical cells.

ABSTRACT: Directed differentiation of pluripotent stem cells into specialized cell types represents an invaluable tool for a wide range of applications. Here, we have exploited single-cell transcriptomic data to develop a stepwise in vitro differentiation system from mouse embryonic stem cells into adrenocortical cells. We show that during development, the adrenal primordium is embedded in an extracellular matrix containing tenascin and fibronectin. Culturing cells on fibronectin during differentiation increased the expression of the steroidogenic marker NR5A1. Furthermore, 3D cultures in the presence of protein kinase A (PKA)-pathway activators led to the formation of aggregates composed of different cell types expressing adrenal progenitor or steroidogenic markers, including the adrenocortical-spe

SUBMITTER: Oikonomakos I 

PROVIDER: S-EPMC11411339 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature