Robust Double Emulsions for Multicolor Fluorescence-Activated Cell Sorting.

ABSTRACT: Cell-cell interactions are essential for the proper functioning of multicellular organisms. For example, T cells interact with antigen-presenting cells (APCs) through specific T-cell receptor (TCR)-antigen interactions during an immune response. Fluorescence-activated droplet sorting (FADS) is a high-throughput technique for efficiently screening cellular interaction events. Unfortunately, current droplet sorting instruments have significant limitations, most notably related to analytical throughput and complex operation. In contrast, commercial fluorescence-activated cell sorters offer superior speed, sensitivity, and multiplexing capabilities, although their use as droplet sorters is poorly defined and underutilized. Herein, we present a universally applicable and simple-to-implement wor

SUBMITTER: Ding Y 

PROVIDER: S-EPMC11411495 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature