Modeling X chromosome inactivation using t5iLA naive human pluripotent stem cells.

ABSTRACT:

Background

X chromosome inactivation (XCI) is a critical epigenetic event for dosage compensation of X-linked genes in female mammals, ensuring developmental stability. A robust in vitro model is required for mimicking XCI during the early stages of embryonic development. This methodology article introduces an advanced framework for the in-depth study of XCI using human pluripotent stem cells (hPSCs). By focusing on the transition between naive and primed pluripotent states, we highlight the role of long non-coding RNA X-inactive specific transcript (XIST) and epigenetic alterations in mediating XCI.

Results

Our methodology enables the distinction between naive and primed hESCs based on XIST expression and the activity of X-linked reporters, facilitating the investigation of

SUBMITTER: Shang Y 

PROVIDER: S-EPMC11411763 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature