VIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters.

ABSTRACT:

Background

Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment.

Methods

A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR. Strains carrying genes of interest were characterized by testing antimicrobial susceptibility, carbapenemase production and combination of short- and long- read whole-genome sequencing. The phylogenetic tree including publicly available genomes of Enterobacter

SUBMITTER: Sukkar I 

PROVIDER: S-EPMC11411806 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature