Physiological significance of the two isoforms of initiator tRNAs in <i>Escherichia coli</i>.

ABSTRACT: Escherichia coli possesses four initiator tRNA (i-tRNA) genes, three of which are present together as metZWV and the fourth one as metY. In E. coli B, all four genes (metZWV and metY) encode i-tRNA^fMet1, in which the G at position 46 is modified to m⁷G46 by TrmB (m⁷G methyltransferase). However, in E. coli K, because of a single-nucleotide polymorphism, metY encodes a variant, i-tRNA^fMet2, having an A in place of m⁷G46. We generated E. coli strains to explore the importance of this polymorphism in i-tRNAs. The strains were sustained either on metY_A46 (metY of E. coli K origin encoding i-tRNA^fMet2) or its derivative metY

SUBMITTER: Sahu AK 

PROVIDER: S-EPMC11411947 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature