PROTAC-mediated vimentin degradation promotes terminal erythroid differentiation of pluripotent stem cells.

ABSTRACT:

Background

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), can undergo erythroid differentiation, offering a potentially invaluable resource for generating large quantities of erythroid cells. However, the majority of erythrocytes derived from hPSCs fail to enucleate compared with those derived from cord blood progenitors, with an unknown molecular basis for this difference. The expression of vimentin (VIM) is retained in erythroid cells differentiated from hPSCs but is absent in mature erythrocytes. Further exploration is required to ascertain whether VIM plays a critical role in enucleation and to elucidate the underlying mechanisms.

Methods

In this study, we established a hESC line with revers

SUBMITTER: Yan H 

PROVIDER: S-EPMC11412063 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature