Evaluation of commercial RNA extraction kits for long-read metatranscriptomics in soil.

ABSTRACT: Metatranscriptomic analysis of the soil microbiome has the potential to reveal molecular mechanisms that drive soil processes regulated by the microbial community. Therefore, RNA samples must be of sufficient yield and quality to robustly quantify differential gene expression. While short-read sequencing technology is often favoured for metatranscriptomics, long-read sequencing has the potential to provide several benefits over short-read technologies. The ability to resolve complete transcripts on a portable sequencing platform for a relatively low capital expenditure makes Oxford Nanopore Technology an attractive prospect for addressing many of the challenges of soil metatranscriptomics. To fully enable long-read metatranscriptomic analysis of the functional molecular pathways expressed

SUBMITTER: Barber DG 

PROVIDER: S-EPMC11412367 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature