Altered Cell Clusters and Upregulated Aqp1 in Connexin 50 Knockout Lens Epithelium.

ABSTRACT:

Purpose

To characterize the heterogeneity and cell clusters of postnatal lens epithelial cells (LECs) and to investigate the downstream targets of connexin 50 (Cx50) in the regulation of lens homeostasis and lens growth. To determine differentially expressed genes (DEGs) in the connexin 50 knockout (Cx50KO) lens epithelial cells that shed light on novel mechanism underlying the cataract and small size of the Cx50KO lenses.

Methods

Single-cell RNA sequencing (scRNA-seq) of lens epithelial cells isolated from one-month-old Cx50KO and wild-type (WT) mice were performed. Differentially expressed genes were identified, and selected DEGs were further studied by quantitative real-time PCR (RT-qPCR) analysis and Western blot analysis.

Results

The expression profiles of severa

SUBMITTER: Xia CH 

PROVIDER: S-EPMC11412383 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature