Exploration of compounds to inhibit the Panton-Valentine leukocidin of Staphylococcus aureus.

ABSTRACT: The Panton-Valentine leukocidin (PVL) of Staphylococcus aureus is associated with necrotizing infections. After binding to complement 5a receptor (C5aR/CD88) and CD45 it causes cytolysis in polymorphonuclear neutrophils (PMNs) as well as inflammasome activation in monocytes. The objective of this study was to test if (ant)agonists of C5aR and CD45 can attenuate the effect of PVL on PMNs and monocytes. We tested the effect of various concentrations of six C5aR (ant)agonists (avacopan, BM213, DF2593A, JPE-1375, PMX205 and W-54011) and one CD45 antagonist (NQ301) to attenuate the cytotoxic effect of PVL on human PMNs and monocytes in vitro. Shifts in the half-maximal effective concentration (EC₅₀) of PVL to achieve a cytotoxic effect on PMNs and modulation of inflammatory cytokine

SUBMITTER: Grebe T 

PROVIDER: S-EPMC11413081 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature