Extracellular matrix production and oxygen diffusion regulate chemotherapeutic response in osteosarcoma spheroids.

ABSTRACT:

Background

Osteosarcoma (OS) survival rates and outcome have not improved in 50 years since the advent of modern chemotherapeutics. Thus, there is a critical need for an improved understanding of the tumor microenvironment to identify better therapies. Extracellular matrix (ECM) deposition and hypoxia are known to abrogate the efficacy of various chemical and cell-based therapeutics. Here, we aim to mechanistically investigate the combinatorial effects of hypoxia and matrix deposition with the use of OS spheroids.

Methods

We use two murine OS cell lines with differential metastatic potential to form spheroids. We form spheroids of two sizes, use ascorbate-2-phosphate supplementation to enhance ECM deposition, and study cell response under standard (21% O₂) and phy

SUBMITTER: Sagheb IS 

PROVIDER: S-EPMC11413413 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature