Dynasore modulates store-operated calcium entry and mitochondrial calcium release in corneal epithelial cells.

ABSTRACT: Dysregulation of calcium homeostasis can precipitate a cascade of pathological events that lead to tissue damage and cell death. Dynasore is a small molecule that inhibits endocytosis by targeting classic dynamins. In a previous study, we showed that dynasore can protect human corneal epithelial cells from damage due to tert-butyl hydroperoxide (tBHP) exposure by restoring cellular calcium (Ca²⁺) homeostasis. Here we report results of a follow-up study aimed at identifying the source of the damaging Ca²⁺. Store-operated Ca²⁺ entry (SOCE) is a cellular mechanism to restore intracellular calcium stores from the extracellular milieu. We found that dynasore effectively blocks SOCE in cells treated with thapsigargin (TG), a small molecule that inhibits pumping o

SUBMITTER: Martinez-Carrasco R 

PROVIDER: S-EPMC11413707 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature