FTO-mediated DSP m⁶A demethylation promotes an aggressive subtype of growth hormone-secreting pituitary neuroendocrine tumors.

ABSTRACT:

Background

Growth hormone-secreting pituitary neuroendocrine tumors can be pathologically classified into densely granulated (DGGH) and sparsely granulated types (SGGH). SGGH is more aggressive and associated with a poorer prognosis. While epigenetic regulation is vital in tumorigenesis and progression, the role of N⁶-methyladenosine (m⁶A) in aggressive behavior has yet to be elucidated.

Methods

We performed m⁶A-sequencing on tumor samples from 8 DGGH and 8 SGGH patients, complemented by a suite of assays including ELISA, immuno-histochemistry, -blotting and -fluorescence, qPCR, MeRIP, RIP, and RNA stability experiments, aiming to delineate the influence of m⁶A on tumor behavior. We further assessed the therapeutic potential of tar

SUBMITTER: Zou Y 

PROVIDER: S-EPMC11414129 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature