Antipolyphosphate monoclonal antibodies derived from autoimmune mice.

ABSTRACT:

Background

Inorganic polyphosphates (polyPs) are linear chains of phosphates that accelerate blood clotting. Targeting polyP in vivo has been shown to reduce thrombosis.

Objectives

To identify and characterize anti-polyP monoclonal antibodies that could be used as analytical tools and as antithrombotic agents.

Methods

Hybridomas were prepared from spleen cells from autoimmune NZBWF1/J female mice and screened for anti-polyP antibodies. Antibodies that bound polyP using enzyme-linked immunosorbent assay and pull-down assays were further characterized with plate binding, surface plasmon resonance, and plasma-based clotting assays. Antithrombotic potential was evaluated in a murine ferric chloride-induced carotid artery thrombosis model.

Results

Of 4 antibo

SUBMITTER: Sedzro JC 

PROVIDER: S-EPMC11414566 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature