Immunopeptidomics Mapping of Listeria monocytogenes T Cell Epitopes in Mice.

ABSTRACT: Listeria monocytogenes is a foodborne intracellular bacterial model pathogen. Protective immunity against Listeria depends on an effective CD8⁺ T cell response, but very few T cell epitopes are known in mice as a common animal infection model for listeriosis. To identify epitopes, we screened for Listeria immunopeptides presented in the spleen of infected mice by mass spectrometry-based immunopeptidomics. We mapped more than 6000 mouse self-peptides presented on MHC class I molecules, including 12 high confident Listeria peptides from 12 different bacterial proteins. Bacterial immunopeptides with confirmed fragmentation spectra were further tested for their potential to activate CD8⁺ T cells, revealing VTYNYINI from the putative cell wall surface anchor family protein

SUBMITTER: Gul A 

PROVIDER: S-EPMC11414675 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature