The branched receptor-binding complex of Ackermannviridae phages promotes adaptive host recognition.

ABSTRACT: Bacteriophages can encode multiple receptor-binding proteins, allowing them to recognize diverse receptors for infecting different strains. Ackermannviridae phages recognize various polysaccharides as receptors by encoding multiple tail spike proteins (TSPs), forming a branched complex. We aimed to mimic the evolution of the TSP complex by studying the acquisition of TSPs without disrupting the complex's functionality. Using kuttervirus S117 as a backbone, we demonstrated that acquiring tsp genes from Kuttervirus and Agtrevirus phages within the Ackermannviridae family led to altered host recognition. A fifth TSP was designed to interact with the branched complex and expand host recognition even further. Interestingly, the acquisition of tsp5 resul

SUBMITTER: Sorensen AN 

PROVIDER: S-EPMC11414711 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature