Frequency-Dependent Engagement of Synaptic Vesicle Pools in the Mice Motor Nerve Terminals.

ABSTRACT: Nerve terminals contain numerous synaptic vesicles (SVs) whose exo-endocytic cycling maintains neurotransmitter release. SVs may have different properties, thereby constituting separate pools. However, behavior of SV pools remains elusive in many synapses. To fill this gap, we studied the functioning of SV pools at both low- and higher-frequency stimulations utilizing microelectrode recording and dual-labeling of SVs with FM-dyes at the mice motor nerve terminals. It was found that higher-frequency stimulation caused exocytosis of different kinds of SVs. One type of SVs contributed to exocytosis exclusively at intense activities and their exocytotic rate was depended on the order in which these SVs were recovered by endocytosis. Another type of SVs can sustain the release in response to bo

SUBMITTER: Gafurova CR 

PROVIDER: S-EPMC11415186 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature