Design and validation of a method for evaluating medical device cleanliness by recovering and quantifying residual proteins on stainless plates.

ABSTRACT: We recently reported a method for recovering and quantifying residual proteins bound to surfaces of various medical instruments via thermal coagulation under neutral pH and room temperature. The method effectively recovered and solubilised coagulated proteins at high temperatures in dry and humid conditions, with a protein recovery rate of > 90%. This study validated the previous method by comparing residual protein recovery from test samples using a conventional extraction solution (1% SDS, [pH 11.0]) and proposed solution (1% SDS, 10 mM TCEP, and 10 mM HEPES [pH 7.0]). To mimic soiled medical equipment, pseudo-blood-contaminated stainless steel plates were prepared. Residual protein was recovered using conventional and proposed solutions under varying temperature and humidity conditions.

SUBMITTER: Uematsu M 

PROVIDER: S-EPMC11415368 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature