Multiple routes to fungicide resistance: Interaction of Cyp51 gene sequences, copy number and expression.

ABSTRACT: We examined the molecular basis of triazole resistance in Blumeria graminis f. sp. tritici (wheat mildew, Bgt), a model organism among powdery mildews. Four genetic models for responses to triazole fungicides were identified among US and UK isolates, involving multiple genetic mechanisms. Firstly, only two amino acid substitutions in CYP51B lanosterol demethylase, the target of triazoles, were associated with resistance, Y136F and S509T (homologous to Y137F and S524T in the reference fungus Zymoseptoria tritici). As sequence variation did not explain the wide range of resistance, we also investigated Cyp51B copy number and expression, the latter using both reverse transcription-quantitative PCR and RNA-seq. The second model for resistance involved higher copy number and expression in isola

SUBMITTER: Arnold CJ 

PROVIDER: S-EPMC11415427 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature