Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways.

ABSTRACT:

Objective

Evaluation of Nepeta cataria as a host with specific endogenous metabolite background for transient expression and metabolic engineering of secondary biosynthetic sequences.

Results

The reporter gene gfp::licBM3 as well as three biosynthetic genes leading to the formation of the cannabinoid precursor olivetolic acid were adopted to the modular cloning standard GoldenBraid, transiently expressed in two chemotypes of N. cataria and compared to Nicotiana benthamiana. To estimate the expression efficiency in both hosts, quantification of the reporter activity was carried out with a sensitive and specific lichenase assay. While N. benthamiana exhibited lichenase activity of 676 ± 94 μmol g^-1 s^-1, N. cataria cultivar '1000', and the cultivar 'Citrio

SUBMITTER: Geissler M 

PROVIDER: S-EPMC11415451 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature