The COL7A1/PI3K/AKT axis regulates the progression of cholangiocarcinoma.

ABSTRACT:

Background

The role and molecular mechanisms of collagen type VII (COL7A1) in cholangiocarcinoma (CCA) remain unknown.

Methods

We analyzed the expression of COL7A1 in CCA and its relationship with patient prognosis using bioinformatic techniques. Expression levels of COL7A1 in CCA cells and tissues were detected using reverse transcription-quantitative PCR, western blotting, and immunohistochemistry. The effects of COL7A1 expression on the proliferation, migration, and invasion of CCA cells were assessed using CCK-8, colony formation, and Transwell assays. Bioinformatics and luciferase reporter gene assays were performed to examine the binding of KLF4 to COL7A1, and cytological experiments further verified the role of KLF4 in regulating the CCA phenotype through COL7A1. Xeno

SUBMITTER: Ma Y 

PROVIDER: S-EPMC11415666 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature