Enhancing terminal erythroid differentiation in human embryonic stem cells through TRIB3 overexpression.

ABSTRACT: Tribbles pseudokinase 3 (TRIB3) expression significantly increases during terminal erythropoiesis in vivo. However, we found that TRIB3 expression remained relatively low during human embryonic stem cell (hESC) erythropoiesis, particularly in the late stage, where it is typically active. TRIB3 was expressed in megakaryocyte-erythrocyte progenitor cells and its low expression was necessary for megakaryocyte differentiation. Thus, we proposed that the high expression during late stage of erythropoiesis could be the clue for promotion of maturation of hESC-derived erythroid cells. To our knowledge, the role of TRIB3 in the late stage of erythropoiesis remains ambiguous. To address this, we generated inducible TRIB3 overexpression hESCs, named TRIB3^{tet-on OE} H9, based on a Tet-On sy

SUBMITTER: Wang X 

PROVIDER: S-EPMC11415673 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature