Single-cell RNA sequencing reveals intratumoral heterogeneity and multicellular community in primary hepatocellular carcinoma underlying microvascular invasion.

ABSTRACT:

Background

Microvascular invasion (MVI) is associated with an unfavorable prognosis and early recurrence of hepatocellular carcinoma (HCC), which is the crucial pathological hallmark of immunotherapy. While microvascular invasion (MVI) in hepatocellular carcinoma (HCC) currently lacks a detailed single-cell analysis of the tumor microenvironment (TME), it holds significant promise for immunotherapy using immune checkpoint inhibitors (ICI).

Methods

We performed single-cell RNA sequencing (scRNA-seq) on 3 MVI positive (MVIP) and 14 MVI-negative (MVIN) tumor tissues, as well as their paired adjacent non-tumoral tissues.

Results

We identified SPP1⁺ macrophages and CD4⁺ proliferative T cells as intertumoral populations critical for the formation of co

SUBMITTER: Sun Z 

PROVIDER: S-EPMC11415683 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature