YTHDF1 in periaqueductal gray inhibitory neurons contributes to morphine withdrawal responses in mice.

ABSTRACT:

Background

Physical symptoms and aversion induced by opioid withdrawal strongly affect the management of opioid addiction. YTH N6-methyladenosine (m⁶A) RNA binding protein 1 (YTHDF1), an m⁶A-binding protein, from the periaqueductal gray (PAG) reportedly contributes to morphine tolerance and hyperalgesia. However, the role of YTHDF1 in morphine withdrawal remains unclear.

Methods

A naloxone-precipitated morphine withdrawal model was established in C57/BL6 mice or transgenic mice. YTHDF1 was knocked down via adeno-associated virus transfection. Combined with the results of the single-cell RNA sequencing analysis, the changes in morphine withdrawal somatic signs and conditioned place aversion (CPA) scores were compared when YTHDF1 originating from differen

SUBMITTER: Ou C 

PROVIDER: S-EPMC11416010 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature