Establishment and validation of circulating cell-free DNA signatures for nasopharyngeal carcinoma detection.

ABSTRACT:

Background

Early detection of nasopharyngeal carcinoma (NPC) poses a significant challenge. The absence of highly sensitive and specific diagnostic biomarkers for nasopharyngeal carcinoma contributes to the unfavourable prognosis of NPC patients. Here, we aimed to establish a non-invasive approach for detecting NPC using circulating cell-free DNA (cfDNA).

Methods

We investigated the potential of next-generation sequencing (NGS) of peripheral blood cells as a diagnostic tool for NPC. We collected data on genome-wide nucleosome footprint (NF), 5'-end motifs, fragmentation patterns, CNV information, and EBV content from 553 Chinese subjects, including 234 NPC patients and 319 healthy individuals. Through case-control analysis, we developed a diagnostic model for NPC, and valida

SUBMITTER: Qiu SF 

PROVIDER: S-EPMC11416236 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature