Deciphering the cellular and molecular landscapes of Wnt/β-catenin signaling in mouse embryonic kidney development.

ABSTRACT:

Background

The Wnt/β-catenin signaling pathway is critical in kidney development, yet its specific effects on gene expression in different embryonic kidney cell types are not fully understood.

Methods

Wnt/β-catenin signaling was activated in mouse E12.5 kidneys in vitro using CHIR99021, with RNA sequencing performed afterward, and the results were compared to DMSO controls (dataset GSE131240). Differential gene expression in ureteric buds and cap mesenchyme following pathway activation (datasets GSE20325 and GSE39583) was analyzed. Single-cell RNA-seq data from the Mouse Cell Atlas was used to link differentially expressed genes (DEGs) with kidney cell types. β-catenin ChIP-seq data (GSE39837) identified direct transcriptional targets.

Results

Activation of Wnt

SUBMITTER: Zhao H 

PROVIDER: S-EPMC11416353 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature