The conformation of tetraspanins CD53 and CD81 differentially affects their nanoscale organization and interaction with their partners.

ABSTRACT: Tetraspanins, including CD53 and CD81, are four-transmembrane proteins that affect the membrane organization to regulate cellular processes including migration, proliferation, and signaling. However, it is unclear how the organizing function of tetraspanins is regulated at the molecular level. Here, we investigated whether recently proposed "open" and "closed" conformations of tetraspanins regulate the nanoscale organization of the plasma membrane of B cells. We generated conformational mutants of CD53 (F44E) and CD81 (4A, E219Q) that represent the "closed" and "open" conformation, respectively. Surface expression of these CD53 and CD81 mutants was comparable to that of WT protein. Localization of mutant tetraspanins into nanodomains was visualized by super-resolution direct stochastic opt

SUBMITTER: Schwerdtfeger F 

PROVIDER: S-EPMC11416636 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature