Protocol to generate two distinct standard-of-care murine glioblastoma models for evaluating novel combination therapies.

ABSTRACT: In cancer research, murine models play a crucial role as highly valuable preclinical tools. Here, we present a protocol to generate a murine model of glioblastoma through the direct intracranial injection of tumor cells. We describe steps for cell culture, intracranial implantation, and standard-of-care treatments. We then detail procedures for monitoring tumor growth using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Pelizzari-Raymundo et al.¹.

SUBMITTER: Pineau R 

PROVIDER: S-EPMC11416638 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature