KA-mediated excitotoxicity induces neuronal ferroptosis through activation of ferritinophagy.

ABSTRACT:

Objectives

This study aims to elucidate the role of Fe²⁺ overload in kainic acid (KA)-induced excitotoxicity, investigate the involvement of ferritinophagy selective cargo receptor NCOA4 in the pathogenesis of excitotoxicity.

Methods

Western blotting was used to detect the expression of FTH1, NCOA4, Lamp2, TfR, FPN, and DMT1 after KA stereotaxic injection into the unilateral striatum of mice. Colocalization of Fe²⁺ with lysosomes in KA-treated primary cortical neurons was observed by using confocal microscopy. Desferrioxamine (DFO) was added to chelate free iron, a CCK8 kit was used to measure cell viability, and the Fe²⁺ levels were detected by FerroOrange. BODIPY C11 was used to determine intracellular lipid reactive oxygen species (ROS) le

SUBMITTER: Jiang YY 

PROVIDER: S-EPMC11416743 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature