The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius.

ABSTRACT:

Introduction

Among hyperthermophilic organisms, in vivo protein localization is challenging due to the high growth temperatures that can disrupt proper folding and function of mostly mesophilic-derived fluorescent proteins. While protein localization in the thermophilic model archaeon S. acidocaldarius has been achieved using antibodies with fluorescent probes in fixed cells, the use of thermostable fluorescent proteins for live imaging in thermophilic archaea has so far been unsuccessful. Given the significance of live protein localization in the field of archaeal cell biology, we aimed to identify fluorescent proteins for use in S. acidocaldarius.

Methods

We expressed various previously published and optimized thermostable fluorescent proteins along wi

SUBMITTER: Recalde A 

PROVIDER: S-EPMC11416942 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature