Characterization of mesothelin gene expression in dogs and overexpression in canine mesotheliomas.

ABSTRACT:

Introduction

Canine mesotheliomas are uncommon malignant tumors typically detected late. Minimally invasive diagnostic biomarkers would facilitate diagnosis at earlier stages, thereby improving clinical outcomes. We hypothesized that mesothelin could be used as a reliable diagnostic biomarker for canine mesotheliomas since it has been used as a cancer biomarker for human mesothelioma. We aimed to explore and characterize mesothelin gene expression in dogs and assess its use as a diagnostic biomarker for canine mesotheliomas.

Materials and methods

We quantified expressed canine mesothelin transcripts via reverse transcription polymerase chain reaction (RT-PCR) and sequenced them using ribonucleic acid (RNA) extracted from a canine mesothelioma cell line. After confirming meso

SUBMITTER: Nabeta R 

PROVIDER: S-EPMC11417096 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature