Single-cell exome sequencing reveals polyclonal seeding and TRPS1 mutations in colon cancer metastasis.

ABSTRACT: Liver metastasis remains the primary cause of mortality in patients with colon cancer. Identifying specific driver gene mutations that contribute to metastasis may offer viable therapeutic targets. To explore clonal evolution and genetic heterogeneity within the metastasis, we conducted single-cell exome sequencing on 150 single cells isolated from the primary tumor, liver metastasis, and lymphatic metastasis from a stage IV colon cancer patient. The genetic landscape of the tumor samples revealed that both lymphatic and liver metastases originated from the same region of the primary tumor. Notably, the liver metastasis was derived directly from the primary tumor, bypassing the lymph nodes. Comparative analysis of the sequencing data for individual cell pairs within different tumors demons

SUBMITTER: Cai J 

PROVIDER: S-EPMC11417107 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature