Variation of Site-Specific Glycosylation Profiles of Recombinant Influenza Glycoproteins.

ABSTRACT: This work presents a detailed determination of site-specific N-glycan distributions of the recombinant influenza glycoproteins hemagglutinin (HA) and neuraminidase. Variation in glycosylation among recombinant glycoproteins is not predictable and can depend on details of the biomanufacturing process as well as details of protein structure. In this study, recombinant influenza proteins were analyzed from eight strains of four different suppliers. These include five HA and three neuraminidase proteins, each produced from a HEK293 cell line. Digestion was conducted using a series of complex multienzymatic methods designed to isolate glycopeptides containing single N-glycosylated sites. Site-specific glycosylation profiles of intact glycopeptides were produced using a recently developed method

SUBMITTER: Goecker ZC 

PROVIDER: S-EPMC11417209 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature