Efficient circularization of protein-encoding RNAs via a novel cis-splicing system.

ABSTRACT: Circular RNAs (circRNAs) have emerged as a promising alternative to linear mRNA, owing to their unique properties and potential therapeutic applications, driving the development of novel approaches for their production. This study introduces a cis-splicing system that efficiently produces circRNAs by incorporating a ribozyme core at one end of the precursor, thereby eliminating the need for additional spacer elements between the ribozyme and the gene of interest (GOI). In this cis-splicing system, sequences resembling homologous arms at both ends of the precursor are crucial for forming the P9.0 duplex, which in turn facilitates effective self-splicing and circularization. We demonstrate that the precise recognition of the second transesterification site depends more on the structural char

SUBMITTER: Qi S 

PROVIDER: S-EPMC11417360 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature