Pseudomonas aeruginosa exotoxin A as a novel allergen induced Non-T_H2 inflammation in a murine model of steroid-insensitive asthma.

ABSTRACT:

Background

Despite the immediate in vivo occurrence of anaphylactic and allergic reactions following treatment with Pseudomonas aeruginosa exotoxin A (PEA)-based immunotoxins, the immunological role of PEA in asthma pathogenesis remains unclear.

Objective

This study investigated the allergenic potential of PEA and the specific type of asthma induced.

Methods

Recombinant PEA (rPEA) lacking domain Ia (to eliminate non-specific cytotoxicity) was expressed, purified, and employed to detect serum PEA-specific IgE levels in asthmatic patients. Competitive ELISA assays were used to assess rPEA's IgE binding capacity and allergenicity. Additionally, rPEA-challenged C57BL/6 mice were subjected to inflammatory endotyping and therapeutic assays to characterize the

SUBMITTER: Xie H 

PROVIDER: S-EPMC11417555 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature